Our lab focuses on the development, maturation, differentiation, phenotype and function of T cells, with particular focus on patients with inborn errors of immunity (IEI) affecting the thymus. We enjoy working with high-parametric and spectral flow cytometry, bulk and single cell RNA sequencing. We utilize modern bioinformatics to analyse large datasets and extract information from clinical data.
Members
Assoc. Prof. Adam Klocperk, MD, PhD
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principal investigator, group leader, consultant |
Prof. Anna Sediva, MD, PhD
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honorary investigator, senior consultant |
Ondrej Vladyka, MD
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MD-PhD student, focus on congenital and acquired defects of thymic development and function, high-parametric and -omics data analysis |
Martin Orlicky, MD
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MD-PhD student, focus on congenital defects of thymic development and function and DNA repair syndromes, in vitro functional analyses |
Irena Zentsova
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staff scientist, innate immunity and dendritic cells |
Katarina Simonová
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undergraduate student, focus on 22q11.2 deletion syndrome, clinical phenotype integration, complex care coordination |
Collaborations
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| AI in education | HPO ontology | IEI annotation | ataxia telangiectasia, ASO therapy |
thymus transplants | T cells, thymus, DNA repair |
neonatal SCID screening, IGLL1 def. |
Publications
2025
Blom, M., Duintjer, A. J., Jamee, M., de Gier, M., Bloomfield, M., Klocperk, A., Kralickova, P., Karaca, N. E., Boyarchuk, O., Čižnár, P., Jeseňák, M., Sharapova, S., Skopovets, E., Gonzalez-Granado, L. I., Palmeri, S., Volpi, S., Nalda, A. M., Tello, S. R., Soler-Palacín, P., … van der Burg, M. (2025). Self-reported clinical outcomes and quality of life in agammaglobulinemia: The importance of an early diagnosis. Journal of Clinical Immunology, 45(1), 125.
Bloomfield, M., Hlaváčková, E., Schneiderová, H., Turnovec, M., Tichý, L., Čech, Z., Chrastina, P., Dvořáková, L., Pešková, K., Formánková, R., Říha, P., Vlková, M., Bejdák, P., Havlišová, M., Froňková, E., Kalina, T., Bíly, V., Říčná, D., Grombiříková, H., … Klocperk, A. (2025). Beyond TREC: Pivotal role of tandem TREC/KREC assay in Czech SCID NBS pilot programme. Pediatric Allergy and Immunology: Official Publication of the European Society of Pediatric Allergy and Immunology, 36(5), e70100.
Friedmann, D., Payne, K. J., Cousin, V., Andrieux, G., Meng, K., Schlaak, A. E., Unger, S., Klocperk, A., Geier, C., Gräwe, K., Pfeiffer, J., Jakob, T. F., Hausmann, O., Anhut, P., Shabani, M., Kurowski, K., Rogg, M., Aumann, K., Seidl, M., … Warnatz, K. (2025). Expansion of a distinct cytotoxic CD4 TFH-cell cluster in lymph nodes of patients with complicated common variable immunodeficiency. The Journal of Allergy and Clinical Immunology. https://doi.org/10.1016/j.jaci.2025.09.032
Golwala, Z. M., Goncalves, H. S., Moirangthem, R. D., Evans, G., Lizot, S., de Koning, C., Garrigue, A., Corredera, M. M., Ocampo-Godinez, J. M., Howley, E., Kricke, S., Awuah, A., Obiri-Yeboa, I., Rai, R., Sebire, N., Bernard, F., De Braem, V. B. C., Boztug, K., Cole, T., … Kreins, A. Y. (2025). Ex vivo T-lymphopoiesis assays assisting corrective treatment choice for genetically undefined T-lymphocytopaenia. Clinical Immunology (Orlando, Fla.), 274(110453), 110453.
Grombirikova, H., Markocsy, A., Kocurkova, A., Blatny, J., Vlkova, M., Slanina, P., Hlavackova, E., Fiamoli, V., Schneiderova, H., Klocperk, A., Ricna, D., Fronkova, E., Kral, J., Salingova, A., Jesenak, M., & Freiberger, T. (2025). Atypical Phenotype of Predominant Autoimmune Cytopenia and Impaired Perforin Expression in XMEN Syndrome. Journal of Immunology Research, 2025(1). https://doi.org/10.1155/jimr/3161910
Kindle, G., Alligon, M., Albert, M. H., Buckland, M., Edgar, J. D., Gathmann, B., Ghosh, S., Gkantaras, A., Nieters, A., Pignata, C., Robinson, P. N., Rusch, S., Schuetz, C., Sharapova, S., Shillitoe, B., Candotti, F., Cant, A. J., Casanova, J.-L., Etzioni, A., … Seidel, M. G. (2025). Inborn errors of immunity: Manifestation, treatment, and outcome—an ESID registry 1994–2024 report on 30,628 patients. Journal of Human Immunity, 1(3). https://doi.org/10.70962/jhi.20250007
Rekerle, L., Danis, D., Rehburg, F., Graefe, A. S. L., Bily, V., Caballero-Oteyza, A., Cacheiro, P., Chimirri, L., Chong, J. X., Connelly, E., de Vries, B. B. A., Dingemans, A. J. M., Duyzend, M. H., Freiberger, T., Gehle, P., Groza, T., Hansen, P., Jacobsen, J. O. B., Klocperk, A., … Robinson, P. N. (2026). GA4GH phenopacket-driven characterization of genotype-phenotype correlations in Mendelian disorders. American Journal of Human Genetics, 113. https://doi.org/10.1016/j.ajhg.2025.12.001
Vladyka, O., Simonová, A. K., Orlický, M., Šedivá, A., & Klocperk, A. (2025). Advances in the management of patients with defects of thymic development. Alergie, 2025(4). https://www.tigis.cz/images/stories/Alergie/Alergie_2025/Alergie_4_2025…
2024
Bloomfield, M., Klocperk, A., Schneiderová, H., Hlaváčková, E., Turnovec, M., Tichý, L., Čech, Z., Chrastina, P., Dvořáková, L., Pešková, K., Formánková, R., Vlková, M., Bejdák, P., Kalina, T., Froňková, E., Bíly, V., Říčná, D., Grombiříková, H., Zachová, R., … Šedivá, A. (2024). The expansion of national newborn screening marks an advancement in diagnosing patients with severe inborn errors of immunity. Ceskoslovenska Pediatrie, 79(3), 136–141.
Danis, D., Bamshad, M. J., Bridges, Y., Caballero-Oteyza, A., Cacheiro, P., Carmody, L. C., Chimirri, L., Chong, J. X., Coleman, B., Dalgleish, R., Freeman, P. J., Graefe, A. S. L., Groza, T., Hansen, P., Jacobsen, J. O. B., Klocperk, A., Kusters, M., Ladewig, M. S., Marcello, A. J., … Robinson, P. N. (2024). A corpus of GA4GH phenopackets: case-level phenotyping for genomic diagnostics and discovery. HGG Advances, 100371, 100371.
Howley, E., Golwala, Z., Buckland, M., Barzaghi, F., Ghosh, S., Hackett, S., Hague, R., Hauck, F., Holzer, U., Klocperk, A., Koskenvuo, M., Marcus, N., Marzollo, A., Pac, M., Sinclair, J., Speckmann, C., Soomann, M., Speirs, L., Suresh, S., … Kreins, A. Y. (2024). Impact of newborn screening for SCID on the management of congenital athymia. The Journal of Allergy and Clinical Immunology, 153(1), 330–334.
Klocperk, A., Bloomfield, M., Schneiderová, H., Hlaváčková, E., Turnovec, M., Tichý, L., Čech, Z., Chrastina, P., Dvořáková, L., Pešková, K., Formánková, R., Vlková, M., Bejdák, P., Froňková, E., Kalina, T., Říčná, D., Grombiříková, H., Bíly, V., Sedláček, P., … Šedivá, A. (2024). Neonatal screening of severe combined immunodeficiency (SCID) in the Czech Republic. Alergie, 2024(2), 85–89.
Klocperk, A., Říha, P., Formánková, R., Kynčl, M., Šedivá, A., & Sedláček, P. (2024). Resolution of granulomatous lesions in a Nijmegen breakage syndrome patient with severe immunodeficiency after hematopoietic stem cell transplantation. Pediatric Allergy and Immunology: Official Publication of the European Society of Pediatric Allergy and Immunology, 35(9). https://doi.org/10.1111/pai.14247
Kreins, A. Y., Dhalla, F., Flinn, A. M., Howley, E., Ekwall, O., Villa, A., Staal, F. J. T., Anderson, G., Gennery, A. R., Holländer, G. A., Davies, E. G., & European Society for Immunodeficiencies Clinical Working Party. (2024). European Society for Immunodeficiencies guidelines for the management of patients with congenital athymia. The Journal of Allergy and Clinical Immunology, 154(6), 1391–1408.
Marchal, A., Cirulli, E. T., Neveux, I., Bellos, E., Thwaites, R. S., Schiabor Barrett, K. M., Zhang, Y., Nemes-Bokun, I., Kalinova, M., Catchpole, A., Tangye, S. G., Spaan, A. N., Lack, J. B., Ghosn, J., Burdet, C., Gorochov, G., Tubach, F., Hausfater, P., COVID Human Genetic Effort, … Bolze, A. (2024). Lack of association between classical HLA genes and asymptomatic SARS-CoV-2 infection. HGG Advances, 5(3), 100300.
Soomann, M., Bily, V., Elgizouli, M., Kraemer, D., Akgül, G., von Bernuth, H., Bloomfield, M., Brodszki, N., Candotti, F., Förster-Waldl, E., Freiberger, T., Giżewska, M., Klocperk, A., Kölsch, U., Nichols, K. E., Krüger, R., Oak, N., Pac, M., Prader, S., … Trück, J. (2024). Variants in IGLL1 cause a broad phenotype from agammaglobulinemia to transient hypogammaglobulinemia. The Journal of Allergy and Clinical Immunology, 0(0). https://doi.org/10.1016/j.jaci.2024.08.002
Vladyka, O., Zieg, J., Pátek, O., Bloomfield, M., Paračková, Z., Šedivá, A., & Klocperk, A. (2024). Profound T Lymphocyte and DNA Repair Defect Characterizes Schimke Immuno-Osseous Dysplasia. Journal of Clinical Immunology, 44(8), 1–11.
Zentsova, I., Klocperk, A., Bloomfield, M., Kubesova, H., Malcova, H., Cebecauerova, D., Horvath, R., Sediva, A., & Parackova, Z. (2024). Tumor-necrosis factor α-rich environment alters type-I interferon response to viral stimuli in patients with juvenile idiopathic arthritis by altering myeloid dendritic cell phenotype. Clinical Immunology , 110170.
2023
Bartůňková, J., Bloomfield, M., Havlišová, M., Klocperk, A., Kubešová, H., Podrazil, M., Střížová, Z., & Šedivá, A. (2023). News in immunology. Vnitrni Lekarstvi, 69(2), 133–137.
Bronsky, J., Copova, I., Durilova, M., Kazeka, D., Kubat, M., Lerchova, T., Vlckova, E., Mitrova, K., Rataj, M., Klocperk, A., Sediva, A., & Hradsky, O. (2023). Postvaccination Immunogenicity of BNT162b2 SARS-CoV-2 Vaccine and Its Predictors in Pediatric Inflammatory Bowel Disease. Journal of Pediatric Gastroenterology and Nutrition, 76(2), e36–e44.
Klocperk, A., Bloomfield, M., Parackova, Z., Aillot, L., Fremuth, J., Sasek, L., David, J., Fencl, F., Skotnicova, A., Rejlova, K., Magner, M., Hrusak, O., & Sediva, A. (2023). B cell phenotype and serum levels of interferons, BAFF, and APRIL in multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C). Molecular and Cellular Pediatrics, 10(1), 15.
Lee, D., Le Pen, J., Yatim, A., Dong, B., Aquino, Y., Ogishi, M., Pescarmona, R., Talouarn, E., Rinchai, D., Zhang, P., Perret, M., Liu, Z., Jordan, I., Elmas Bozdemir, S., Bayhan, G. I., Beaufils, C., Bizien, L., Bisiaux, A., Lei, W., … Casanova, J.-L. (2023). Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children. Science, 379(6632), eabo3627.
Matuozzo, D., Talouarn, E., Marchal, A., Zhang, P., Manry, J., Seeleuthner, Y., Zhang, Y., Bolze, A., Chaldebas, M., Milisavljevic, B., Gervais, A., Bastard, P., Asano, T., Bizien, L., Barzaghi, F., Abolhassani, H., Abou Tayoun, A., Aiuti, A., Alavi Darazam, I., … Cobat, A. (2023). Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19. Genome Medicine, 15(1), 22.
Parackova, Z., Zentsova, I., Bloomfield, M., Klocperk, A., Horvath, R., Malcova, H., Cebecauerova, D., & Sediva, A. (2023). Expanded population of low-density neutrophils in juvenile idiopathic arthritis. Frontiers in Immunology, 14. https://doi.org/10.3389/fimmu.2023.1229520
Vladyka, O., Vrabcova, P., Reiterova, M., Parackova, Z., Haesler, R., Sediva, A., Kalina, T., & Klocperk, A. (2023). Th1/interferon-γ bias in 22q11.2 deletion syndrome is driven by memory T cells and exacerbated by IL-7. Clinical Immunology , 109793.
Grants and funding
JMF 22q ANTIC: 22q11.2 Deletion Syndrome - Assessing patient Needs and Trajectories in Czechia
AZV NU23-05-00097: ThymIA: Thymic dysfunction and its role in Innate and Adaptive immunity
Awards
Minister of Health Honorary Award 2024
Awarded to prof. Kalina and assoc. prof. Klocperk for the project AZV NU20-05-00282 T-MAPs: High content mapping of surface molecules in normal and disturbed development T lymphocytes, a search for diagnostic and therapeutic targets
Czech society for analytical cytometry award 2024
Awarded to Dr. Vladyka in the category of best publication with the topic of clinical cytometry for the paper Vladyka et al. Th1/interferon-γ bias in 22q11.2 deletion syndrome is driven by memory T cells and exacerbated by IL-7
V. Špičák award 2024
Awarded to Assoc. Prof. Klocperk in the category of best publication in the Alergie journal for the paper Klocperk et al. Neonatal screening of severe combined immunodeficiency (SCID) in the Czech Republic
