Our lab focuses on the development, maturation, differentiation, phenotype and function of T cells, with particular focus on patients with inborn errors of immunity (IEI) affecting the thymus. We enjoy working with high-parametric and spectral flow cytometry, bulk and single cell RNA sequencing. We utilize modern bioinformatics to analyse large datasets and extract information from clinical data.
Members
Assoc. Prof. Adam Klocperk, MD, PhD
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principal investigator, group leader, consultant |
Prof. Anna Sediva, MD, PhD
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honorary investigator, senior consultant |
Ondrej Vladyka, MD
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MD-PhD student, focus on congenital and acquired defects of thymic development and function, high-parametric and -omics data analysis |
Martin Orlicky, MD
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PhD student, focus on congenital defects of thymic development and function and DNA repair syndromes, in vitro functional analyses |
Irena Zentsova
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staff scientist, innate immunity and dendritic cells |
Katarina Simonová
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undergraduate student, focus on 22q11.2 deletion syndrome, clinical phenotype integration, complex care coordination |
Collaborations
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| AI in education | HPO ontology | IEI annotation | ataxia telangiectasia, ASO therapy |
thymus transplants | T cells, thymus, DNA repair |
neonatal SCID screening, IGLL1 def. |
Publications
2025
(submitted) Grombirikova, Journal of Immunology Research: Atypical phenotype of predominant autoimmune cytopenia and impaired perforin expression in XMEN syndrome
(submitted) Danis, Nature Biotechnology: GA4GH Phenopacket-Driven Characterization of Genotype-Phenotype Correlations in Mendelian Disorders
Bloomfield, Pediatric Allergy and Immunology: Beyond TREC: Pivotal Role of Tandem TREC/KREC Assay in Czech SCID NBS Pilot Programme
Golwala, Clinical Immunology: Ex vivo T-lymphopoiesis assays assisting corrective treatment choice for genetically undefined T-lymphocytopaenia
2024
Klocperk, Alergie: Neonatal screening of severe combined immunodeficiency (SCID) in the Czech Republic
Zentsova, Clinical Immunology: Tumor-necrosis factor α-rich environment alters type-I interferon response to viral stimuli in patients with juvenile idiopathic arthritis by altering myeloid dendritic cell phenotype
Bloomfield, Česko-slovenská pediatrie: The expansion of national newborn screening marks an advancement in diagnosing patients with severe inborn errors of immunity
Danis, HGG Advances: A corpus of GA4GH phenopackets: case-level phenotyping for genomic diagnostics and discovery
Marchal, HGG Advances: Lack of association between classical HLA genes and asymptomatic SARS-CoV-2 infection
Kreins, Journal of Allergy and Clinical Immunology: European Society of Immunodeficiencies guidelines for the management of patients with congenital athymia
Howley, Journal of Allergy and Clinical Immunology: Impact of newborn screening for SCID on the management of congenital athymia
Soomann, Journal of Allergy and Clinical Immunology: Variants in IGLL1 cause a broad phenotype from agammaglobulinemia to transient hypogammaglobulinemia
Vladyka, Journal of Clinical Immunology: Profound T Lymphocyte and DNA Repair Defect Characterizes Schimke Immuno-Osseous Dysplasia
Klocperk, Pediatric Allergy and Immunology: Resolution of granulomatous lesions in a Nijmegen breakage syndrome patient with severe immunodeficiency after hematopoietic stem cell transplantation
2023
Vladyka, Clinical Immunology: Th1/interferon-γ bias in 22q11.2 deletion syndrome is driven by memory T cells and exacerbated by IL-7
Parackova, Frontiers in Immunology: Expanded population of low-density neutrophils in juvenile idiopathic arthritis
Matuozzo, Genome Medicine: Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19
Bronsky, Journal of Pediatric Gastroenterology and Nutrition: Postvaccination Immunogenicity of BNT162b2 SARS-CoV-2 Vaccine and Its Predictors in Pediatric Inflammatory Bowel Disease
Klocperk, Molecular and Cellular Pediatrics: B cell phenotype and serum levels of interferons, BAFF, and APRIL in multisystem inflammatory syndrome in children associated with COVID-19 (MIS-C)
Lee, Science: Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children
Bartunkova, Vnitrni lekarstvi: News in immunology
Grants and funding
(Under evaluation) AZV NW26-05-00027: DART: Differentiation patterns, clinical Associations and Rejuvenation of T-cell Immunity in Primary ImmunoDeficiencies
JMF 22q ANTIC: 22q11.2 Deletion Syndrome - Assessing patient Needs and Trajectories in Czechia
AZV NU23-05-00097: ThymIA: Thymic dysfunction and its role in Innate and Adaptive immunity
Awards
Minister of Health Honorary Award 2024
Awarded to prof. Kalina and assoc. prof. Klocperk for the project AZV NU20-05-00282 T-MAPs: High content mapping of surface molecules in normal and disturbed development T lymphocytes, a search for diagnostic and therapeutic targets
Czech society for analytical cytometry award 2024
Awarded to Dr. Vladyka in the category of best publication with the topic of clinical cytometry for the paper Vladyka et al. Th1/interferon-γ bias in 22q11.2 deletion syndrome is driven by memory T cells and exacerbated by IL-7
V. Špičák award 2024
Awarded to Assoc. Prof. Klocperk in the category of best publication in the Alergie journal for the paper Klocperk et al. Neonatal screening of severe combined immunodeficiency (SCID) in the Czech Republic
